Single-cell RNA-seq of Cardiac Neural Crest Cell Derivatives

The migratory cardiac neural crest cells (CNCCs) greatly contribute to the cardiovascular development. A thorough understanding of the cell lineages, transcriptomic states and regulatory networks of the CNCC derivatives during normal development is essential for deciphering the pathogenesis of those CNCC-associated congenital anomalies. However, the transcriptomic landscape of the CNCC derivatives during development have not yet been examined at single-cell resolution.

We performed single-cell transcriptomic sequencing of 34,131 CNCC-derived cells in mouse hearts from eight developmental stages between E10.5 and P7. Our study represents the first systematic interrogation of the transcriptional landscape of the CNCC derivatives during cardiac development at single-cell resolution. Our study provides novel insights into the cell lineages, molecular signature, developmental chronology and state change dynamics of the CNCC derivatives during embryonic and neonatal development. Our dataset constitutes a valuable resource facilitating future efforts in exploring the role of CNCC derivatives played in development and disease.

Gene Expression

Dynamic Change